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Data from: Isolation and characterization of 145 polymorphic microsatellite loci for the common frog (Rana temporaria)

Cite as:

Matsuba, Chikako; Merilä, Juha; (): Data from: Isolation and characterization of 145 polymorphic microsatellite loci for the common frog (Rana temporaria). https://doi.org/10.5061/dryad.8109

2010-12-16T22:28:22.000+0100 Matsuba, Chikako; Merilä, Juha; 10.5061/dryad.8109

We describe primers and polymerase chain reaction conditions to amplify 145 di-, tri- and tetranucleotide microsatellite loci from the common frog (Rana temporaria), a species commonly used as a model in ecological and evolutionary research. Primers were tested on 46 individuals from two Fennoscandian populations and yielded an average of six to nine alleles per locus (range = 1–30) depending on the population. Average observed heterozygosities in the two populations were 0.16 (range = 0–0.91) and 0.36 (range = 0–1).